Serosal esophagus and rectal ion flux in response to mucosal application of inhibitors.

Effect of application of Cortland’s saline containing 0.1mM ouabain, 0.1mM Co3Hex or both inhibitors to the mucosal surface of non-everted esophageal and rectal unfed C. auratus tissue on relative Mg2+ (A), Ca2+ (B) and H+ (C) transport. Values are relative to the control treatment (symmetrical application of Cortland saline). Salines used on both surfaces were buffered with 10mM HEPES (pH = 7.4). All measurements were taken at the serosal surface. Data are mean flux ± S.E.M. N = 6. A repeated measures one-way ANOVA (inhibitor as factor) and post-hoc Holm-Sidak test was used to compare individual treatments to the control (* indicates significant difference (p<0.05) from control).