Additional file 8: of Regulatory and sequence evolution in response to selection for improved associative learning ability in Nasonia vitripennis

Figure S4. Genes with consistently evolved expression differences as identified by three separate methods. A total of 36 genes showed significant evolved expression differences consistent across four replicate pairs of lines. We identified these genes as they were significant outliers in differential expression analysis in edgeR, significantly correlated with the axis separating control and selected lines in a PCA, and / or showed significant differences between the selected and control line within line pairs. In addition, we only retained genes with a twofold or higher absolute expression difference as a result of selection (see Methods). (PDF 19 kb