Additional file 8: of Root mediated uptake of Salmonella is different from phyto-pathogen and associated with the colonization of edible organs

Figure S8. Protocol for separation of rhizoplanic bacteria from internalized bacteria. (A) Scematic representation of the protocol for estimating surface colonizing versus the invading bacteria. (B) Representative confocal image of the entire root before and after vortex. Note that these are same root shown before and after vortex. The same region on both the roots are zoomed in (A and B). Vortex above 200RPM leads to mechanical damage to the root. (C and D) Comparison between the viability of bacteria after vortexing and after 1% sodium hypochloride treatment respectively. Please note that the same root was first imaged after vortexing and then after sodium hypochloride treatment. Arrow head showing the internalized Salmonella. Ep = Epidermis; Co = Cortex. (TIF 2016 kb