Additional file 1: of Root mediated uptake of Salmonella is different from phyto-pathogen and associated with the colonization of edible organs

Figure S1. Growth of bacteria on cellulose and pectin containing media and pattern of colonization on plant roots. (A and B) Growth of Salmonella, Pseudomonas, Ralstonia and Xanthomonas on M9 minimal media with 0.3% cellulose or pectin as the sole carbon source respectively. Tomato roots inoculated with (C) Ralstonia solanacearum (mcherry), (D) Pseudomonas syringae (GFP), (E) Salmonella Typhimurium (GFP) and R. solanacearum ΔhrpB (mcherry). Images were taken after 3 days post infection. Propidium iodide and DAPI is used to stain the plant in (E) and (F) respectively. Pr = Pericycle and En = Endodermis. The dotted line represents the vasculature. (G) CFU of Salmonella and phyto-pathogens in rhizoplane and tissue. One way ANOVA was used to analyze the data. Different alphabets represent significance at p < 0.005. (H) Growth of Salmonella and phyto-pathogens in tomato root exudates. (I) Representative image of Ralstonia solanacearum colonization on arabidopsis. The green arrowhead represents lateral root emerging areas and the white arrowhead represents non emerging areas. Please note that mcherry fluorescence is coming from all over the root (i) specially in the vasculature (ii and iii). (TIF 1310 kb