Cytochrome C release in dissociated bladder tumors.

Graphs representing the percentage of Cytochrome-C-positive cells in dissociated bladder tumors. Tumor cells were cultured for 1–2 days before being subjected to hyperthermia in combination with 40 μg/ml MMC or 40 μg/ml cisplatin. After treatment, medium was refreshed and the caspase-inhibitor Q-VD-OPh was added. After 24 hours, cells were fixed, stained and scored for Cytochrome C-release positivity. A)-H) all represent different tumors. N.d. = not determined. The number of cells analyzed are indicated in each bar, which represent proportion ± standard error of the proportion. Asterisk indicate significance as determine by Students’ t-tests: * pppp-values can be found in S3 Table.