Determination of CYP1 expression by enzyme activity assay.

<p>The X axis corresponds to patient numbers while the Y axis to CYP1 activity levels. Activity was calculated from production of the metabolite luteolin per time per amount of microsomal protein. Each bar represents an average of triplicate reactions. Ta-T1 and T2-T3 correspond to different stages of tumors according to TNM. (A) Bladder samples (n=10). Colon samples (n=8). ns not statistically significant, * statistically different p< 0.05. (B) Mean CYP1 activity levels in human tumors. Bar charts indicate mean ± STDEV for bladder (n=10) and colon (n=8) tumors and normal samples. Statistical analysis was conducted using paired T test and Wilcoxon ranks test. Statistical differences were obtained for bladder and colon tumors vs normals (p<0.05).