Different maternal diet intervention differentially affected the number of macrophages infiltration.

(A) HE staining used to identify CLS (n = 5–7). There was no evident presence of CLS or F4/80 in the REF group. Red arrows indicate CLS. (B) F4/80 staining used to indicate pro-inflammatory macrophages. Green arrows indicate F4/80 present in CLS. Blue arrows indicate F4/80 presence on cell membrane. (C) Absolute percent area of CLS present in visceral adipose tissue determined H9N had a significant decrease compared to both NF and HF groups (n = 5–7, mean ±SEM). (D) Both HF and H5N groups were significantly increased compared to NF group. Data is reported as Mean±SEM, n = 3–5. The “*” symbol indicates P<0.05 versus the NF group. The “#” symbol indicates P<0.05 versus the HF group.