The pro-inflammatory pathways, NF-κB and JNK pathways activation in the offspring adipose tissue was differentially affected by different maternal diet interventions.

(A) The expressions of Phospho-NF-κB (65 kDa), NF-κB (65 kDa), JNK (54 kDa) and Phospho-JNK (54 kDa) were detected by Western Blots (n = 3 per group). (B) The Phospho-NF-κB/ NF-κB ratio was calculated and used for statistical analysis. The expression level was normalized against the REF group. Data is reported as Mean±SEM, n = 3–4. The “*” symbol indicates P<0.05 versus the NF group. The “#” symbol indicates P<0.05 versus the HF group. The “&” symbol indicate P<0.05 versus the REF group. (C) The Phospho-JNK/JNK ratio was calculated and used for statistical analysis. Data is reported as Mean±SEM, n = 3–4. The “*” symbol indicates P<0.05 versus the NF group. The “#” symbol indicates P<0.05 versus the HF group. The “&” symbol indicate P<0.05 versus the REF group.