The chronic effect of 0.15 mmol/L Zn²⁺ on SN56 cells.

Immunostainings with specific antibodies: (A) L-VGCC, (B) P/G-VGCC, (C) N-VGCC, white bar equals 20 μm. Photographs of Western blot membranes: (D) L-VGCC, (E) P/G-VGCC, (F) N-VGCC as well as the quantitation of the band intensity: (G) L-VGCC, (H) P/G-VGCC, (I) N-VGCC. The confirmation of Zn2+ toxicity: (J) total protein level, (K) trypan blue test. Data are means ± SEM from 4–7 experiments. Significantly different from SN56 control (***p<0.0001).