Effect of purification on the binding of mAbs to different ACEs.

ACE activity in any pair of tested sources of ACEs was equilibrated with ZPHL as a substrate and precipitation of ACE activity was performed as in Fig 3. A. The ratio of precipitated activity of pure lung ACE to that of pure plasma ACE. B. Effect of ACE purification from human plasma on mAbs binding. C Effect of ACE purification from human heart homogenate (1:9, tissue:buffer) on mAbs binding. D. Effect of ACE purification o from human lung homogenate (1:9, tissue:buffer) on mAbs binding. Ratios are expressed in % and presented as a mean of at least 3 independent experiments. N/T–not tested mAbs. The coloring is as in the legend to Fig 3.