Conformational fingerprinting of serum ACEs with a set of mAbs to ACE.

<p><b>A</b>. Sixteen monoclonal antibodies were used to precipitate ACE from sera. Immunoprecipitated ACE activity is presented as a normalized value (“binding ratio”), to highlight differences in immunoprecipitation pattern (“conformational fingerprint”) among blood ACE from patient # 27 and blood ACE from healthy individuals (which were expressed as 100%). <b>B</b>. Binding of several mAbs to serum ACE from control samples and serum from patient # 27-relative to binding by mAb 9B9. Data expressed as % of mAbs/9B9 binding ratio for serum from patient # 27 to that for control serum. Bars highlighted with yellow - samples from serum of patient # 27 with values of precipitated ACE activity 20% lower than this value for control sample. Bars highlighted with red- samples from serum of patient # 27 with values of precipitated ACE activity 20% higher than this value for control sample. <b>C</b>. Effect of ACE inhibitor enalaprilat (100 nM) on binding of mAbs 1G12 and 6A12 to serum ACE from control samples and serum from patient # 27. All data presented as a mean of 3–5 independent determinations. * p<0.05 indicates values shown is significantly different.