P28 GST enzymatic activity is essential to decrease inflammation in TNBS-induced colitis.

A, GST activity was measured in the presence of reduced glutathione at pH 6.5. The GST activity of P28GST is significantly increased when compared to Human Placenta (HP) GST. B, Antioxidant activity of intact or heat-inactivated P28GST or HP GST was determined by ABTS radical scavenging activity. C, Clinical scores obtained after curative treatment of Balb/c mice with TNBS. Balb/c mice were treated with 2 injections of either GST from human placenta (GST HP) and intact or heat-inactivated 5μg/kg P28GST. Data are expressed as means ± SEM from three similar experiments. *p<0.05.; **p<0.01; ***p<0.001.