Inflammatory markers are decreased after treatment with P28GST.

Mice were treated with 2 ip injections of either P28GST or methylprednisolone after TNBS-injection. Colons and peripheral blood were collected from each mouse 2 days after TNBS injection. A, MPO was quantified by ELISA in mice colons. MPO levels expressed as ng per mg of total proteins are lower in treated animals. B, Cox-2; C, Tumor necrosis factor (TNF); E, IL-1β and G, IL-6 mRNA expression was assessed by quantitative PCR in mice colons. D, F, H, Pro-inflammatory cytokines (TNF, IL-1β and IL-6) concentrations were investigated in each serum sample. Data are presented as mean value ± SEM of four similar experiments. N = 10–12 per group. *p<0.05; **p<0.01; ***p<0.001.