TLR3 deficiency leads increased rates of macromolecular permeability in OE cell monolayers during <i>Chlamydia</i> infection.

Macromolecular permeability assays were performed in WT and TLR3-deficient OE cell lines that were either mock-infected or C. muridarum-infected at an MOI of 1 IFU/ cell. Relative permeability was measured using a FITC-labeled dextran (70-kDa) probe. Samples were taken from the basolateral chamber of the transwell every 6hrs post-infection, and permeability was determined by increases in relative fluorescence compared to mock-infected controls. Data are representative of three independent experiments. WT = wild-type and TLR3(-) = TLR3-deficient OE cells. The data are represented as mean (SD). Two-way ANOVA with Tukey’s multiple comparisons test applied. *** = p 0.0003 thru 0.0001, **** = p .0001 comparing WT and TLR3-deficient OE cells at the given time.