TLR3 deficiency impacts the chlamydial-induced rearrangement of claudin-1, JAM-1, and ZO-1 in cellular tight junctions.

WT and TLR3-deficient OE cells were infected with a low MOI of 1 IFU/ cell C. muridarum for up to 36 hours before cells were fixed at the listed time-point post infection and examined by immunofluorescence as described in Materials and Methods. The intensity of the TJ fluorescence in the infected cells was differentially regulated throughout infection in the TLR3-deficient OE cells when compared to WT OE cells. OE-WT = wild-type and OE-TLR3(-/-) = TLR3-deficient OE cells. All images are 40x magnification and nuclei were counterstained with DAPI (blue).