Pro-longevity effect of tanespimycin in <i>C</i>. <i>elegans</i>.

A) Representative fluorescent images of day 6 adult, hsp-16.2p::mCherry transcriptional reporter worms, grown on plates containing 0.1% DMSO (vehicle) or different concentrations of tanespimycin (17-AAG) continuously from the first larval stage, or exclusively from the first day of adulthood onward. B) The relative fluorescent intensity of hsp-16.2p::mCherry worms grown on plates containing 0, 1, 10, 25, 50, or 100 μM tanespimycin (17-AAG) continuously from the first larval stage or exclusively from the first day of adulthood onward. Values plotted are the mean of at least 5 animals, and error bars represent the standard deviation from the mean. Statistical significance relative to the DMSO control group was calculated by ONE-WAY ANOVA with Tukey post analysis pairwise comparison of groups. * = p hsp-90(RNAi). Statistical significance was calculated by Log-rank (Mantel-Cox) text. *** = p hsp-90 mRNA levels 48 hours following exposure to empty vector control or hsp-90(RNAi). Levels of hsp-90 mRNA were normalized to the geometric mean of three house-keeping genes (cdc-42, rpb-2, and pmp-3). Values plotted are the mean of 3 biological replicates and error bars represent standard deviation. Significance levels were calculated as in Fig 3B.