Glycine extends the lifespan of <i>C</i>. <i>elegans</i>.

(A) HPLC-MS/MS quantification of glycine in the life history of nematodes fed UV-killed E. coli OP50 from the larval stage 3 (L3) to D9 adult worms. Levels of other amino acids measured from the same batch of samples were indicated in S4A Fig. Mean ± SD with three biological replicates; Significance was calculated using one-way ANOVA; *p p (B) Lifespan analyses of C. elegans cultured on UV-killed E. coli OP50 showing that glycine supplementation significantly extends lifespan at molarities of 5 μM, p p p S1 Table for lifespan statistics. (C) Scheme showing the different timing conditions of glycine treatments. (D) Lifespan analyses of C. elegans fed UV-killed E. coli OP50 and supplemented with dietary glycine at different times in the animal’s life. Glycine supplementation during larval stage only (from the time of hatching to L4 larval stage) has no effect on lifespan (green line, p = 0.18); Glycine supplementation during the first three days of adulthood extends lifespan by 19.4% (yellow line, p p p S1 Table for lifespan statistics. (E) Survival curves for worms subjected to gcst-1 RNAi. Worms were fed HT115 RNAi bacteria expressing dsRNA corresponding to gcst-1 or empty vector controls from the time of hatching. RNAi depletion of gcst-1 does not influence the lifespan of the wild-type N2 C. elegans relative to empty vector controls (p = 0.37). Comparisons of survival curves was performed by log-rank tests. See S1 Table for lifespan statistics. (F) Survival curves for worms subjected to mel-32 RNAi. Worms were fed HT115 RNAi bacteria expressing dsRNA corresponding to mel-32 or empty vector controls from the time of hatching. Knockdown of mel-32 results in a significant lifespan extension compared with empty vector controls (p S1 Table for lifespan statistics.