The pBT1-encoded <i>cysS</i> is required for optimal growth of <i>S</i>. Typhimurium D23580.
- Rocío Canals (595216)
- Disa L. Hammarlöf (529156)
- Carsten Kröger (497893)
- Siân V. Owen (825674)
- Wai Yee Fong (6208262)
- Lizeth Lacharme-Lora (817511)
- Xiaojun Zhu (352546)
- Nicolas Wenner (124607)
- Sarah E. Carden (6208265)
- Jared Honeycutt (174904)
- Denise M. Monack (243058)
- Robert A. Kingsley (146912)
- Philip Brownridge (1342554)
- Roy R. Chaudhuri (6758)
- Will P. M. Rowe (6208268)
- Alexander V. Predeus (2329939)
- Karsten Hokamp (42210)
- Melita A. Gordon (146918)
- Jay C. D. Hinton (95324)
Publication date
January 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Growth curves of D23580 WT, D23580 ΔcysSchr::frt, D23580 pBT1-cured strain, and D23580 ΔcysSpBT1::frt strains in LB medium, n = 8 (standard deviations are represented) (S1 Data). (B) Growth curves in minimal M9 medium supplemented with 0.4% glucose, n = 5 (standard deviations are represented) (S1 Data). (C) Comparison of cysSchr expression levels (TPM values) of 4/74 (n = 3), D23580 (n = 3), and the D23580 pBT1-cured strain (n = 2) in the ESP growth condition (S1 Data). Bars represent mean values and standard deviations. Significant differences (***) indicate p-value S8 Table. ESP, early stationary phase; LB, Lennox broth; ns, not significant; OD, optical density; ST, sequence type; TPM, transcripts per million; WT, wild type.</p
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