[Urea]1/2 and [GdnHCl]1/2 midpoints of different LZ2 mutants as determined by CD spectroscopy.</p
<p>(A) The folded protein in the absence of urea (solid line) compared with the unfolded protein in ...
<p>For each mutant protein at 10 µM concentration in 10 mM phosphate buffer, pH 8.0, an average of t...
<p>Changes in average emission wavelength (AEW) of tryptophan fluorescence are represented as a func...
<p>Urea-induced denaturation of CTD<sub>Mi</sub> mutants. The solid lines represent the fitting curv...
<p>Urea-induced unfolding curves of far-UV CD spectra of CheW wild-type (black) and R62A mutant (red...
<p>(<i>A</i>) Far-UV circular dichroism spectra and (<i>B–C</i>) urea-induced denaturation of wild t...
Circular dichroism (CD) spectra of wild-type and mutants of zinc finger motifs in Cas12g.</p
<p>A. Stx1B wild-type and L41Q mutant CD wavelength spectra. The far-UV spectra overlay, suggesting ...
<p>100 µl of protein at 10 µg/mL was incubated for 16 h in 1.5 M urea, in the absence or the presenc...
<p>(<b>A</b>) Overlay of CD spectra from Ldb1-Lhx3 (<i>solid black squares</i>) and Ldb1-Lhx3(Y114C)...
<p>CD spectroscopy was used to estimate the secondary structures of individual mutants. The CD signa...
<p>The enzymes were preincubated in various urea concentrations in 30 mM Tris-acetate (pH 7.4) at 25...
<p>The relative specificity constants for methylation of the 29 nt mutants.</p><p>The relative speci...
<p>Comparison of CD spectra of wild-type BglTM and N31C-T187C/P102C-N125C mutant in 20 mM phosphate ...
<p>A. NDAPH level detected in control cells, IDH1 wild type stable cells and IDH1 R132H mutant stabl...
<p>(A) The folded protein in the absence of urea (solid line) compared with the unfolded protein in ...
<p>For each mutant protein at 10 µM concentration in 10 mM phosphate buffer, pH 8.0, an average of t...
<p>Changes in average emission wavelength (AEW) of tryptophan fluorescence are represented as a func...
<p>Urea-induced denaturation of CTD<sub>Mi</sub> mutants. The solid lines represent the fitting curv...
<p>Urea-induced unfolding curves of far-UV CD spectra of CheW wild-type (black) and R62A mutant (red...
<p>(<i>A</i>) Far-UV circular dichroism spectra and (<i>B–C</i>) urea-induced denaturation of wild t...
Circular dichroism (CD) spectra of wild-type and mutants of zinc finger motifs in Cas12g.</p
<p>A. Stx1B wild-type and L41Q mutant CD wavelength spectra. The far-UV spectra overlay, suggesting ...
<p>100 µl of protein at 10 µg/mL was incubated for 16 h in 1.5 M urea, in the absence or the presenc...
<p>(<b>A</b>) Overlay of CD spectra from Ldb1-Lhx3 (<i>solid black squares</i>) and Ldb1-Lhx3(Y114C)...
<p>CD spectroscopy was used to estimate the secondary structures of individual mutants. The CD signa...
<p>The enzymes were preincubated in various urea concentrations in 30 mM Tris-acetate (pH 7.4) at 25...
<p>The relative specificity constants for methylation of the 29 nt mutants.</p><p>The relative speci...
<p>Comparison of CD spectra of wild-type BglTM and N31C-T187C/P102C-N125C mutant in 20 mM phosphate ...
<p>A. NDAPH level detected in control cells, IDH1 wild type stable cells and IDH1 R132H mutant stabl...
<p>(A) The folded protein in the absence of urea (solid line) compared with the unfolded protein in ...
<p>For each mutant protein at 10 µM concentration in 10 mM phosphate buffer, pH 8.0, an average of t...
<p>Changes in average emission wavelength (AEW) of tryptophan fluorescence are represented as a func...