MOESM7 of CIB1 depletion with docetaxel or TRAIL enhances triple-negative breast cancer cell death

Additional file 7: Figure S6. CIB1 depletion may upregulate TRAIL-R1/R2 and IGF-1R expression in docetaxel-resistant TNBC cells. CIB1 depletion potentiates TRAIL-induced cell death in docetaxel-resistant MDA-436 cells potentially via upregulation of both TRAIL-R1 and –R2. a) Dose-response of docetaxel-induced cell death in parental (MDA-436-PR) versus docetaxel-resistant (MDA-436-DCXR) TNBC cells over 48 hr confirms resistance in MDA-436-DCXR cells. Cell death was quantified using trypan blue exclusion assay. Data represents means +/- SD (n=2). FACS analysis of cell surface expression of b) TRAIL-R1 and c) TRAIL-R2 in CIB1 depleted (shCIB1) MDA-436-PR and MDA-436-DCXR cells normalized to IgG-stained control cells (shCTRL) 4 days post infection with RNA interference. Data represent means +/- SD (n=3); * P < 0.05; ** P < 0.01. d) Representative Western blot from 3 separate experiments showing TRAIL-R1, TRAIL-R2, and vinculin (loading control) expression in MDA-436-PR and MDA-436-DCXR cells 3 and 4 days post-infection with either shControl (Ctr) or shCIB1 (CIB1). e) Paraptotic signaling in a chemo-resistant setting was analyzed by probing for IGF-1R, phosphor-JNK, total JNK, and Rac (loading control) in control or CIB1-depleted parental and docetaxel-resistant TNBC cells treated with either docetaxel (10 nM) or TRAIL (5 ng/mL) for 48 h (n=2)