Reprogramming of <i>Trypanosoma cruzi</i> metabolism triggered by parasite interaction with the host cell extracellular matrix - Fig 4

Enzymatic activity of three enzymes from the glucose metabolism: HK (A) PK (B) and LDH (C). (a) Quantification of the respective enzyme activity in extracts of trypomastigotes of T. cruzi incubated (MTy) or not (Ty) with ECM and expressed for 1 x 108 parasites. (b) Loading control of PAR (Paraflagellar-rod proteins) for the three replicates used in the measure of each enzyme activity: HK (A), PK (B) and LHD (C). (c) Quantification of the enzyme activity of extracts from trypomastigotes incubated (Ty_AP) or not (Ty) with alkaline phosphatase. (d) Relative quantification of enzyme activity of extracts of trypomastigotes incubated with ECM followed by incubation (MTy_AP) or control (Ty) with alkaline phosphatase and expressed as percentage of activity. The number of parasites (Fig 4a) was based on the calibration curve presented in S3 Fig.