Targeted DamID of control and <i>fuss</i> mutants reveal sensory neuron markers as potential Fuss targets.

(A) A Dam-PolII/Dam binding pattern was generated from nine individual TaDa profiles and averaged to one single track. fuss (red box) and toy (blue box) are highly covered by Dam-PolII. Regions bound stronger by Dam-PolII than by Dam are depicted in green. Regions bound stronger by Dam than by Dam-PolII are depicted in red. (B) Verification of three TaDa positive genes, toy, atonal and acj6 by immunostaining. Toy was labelled by anti-Toy (green) and Fuss by anti-Fuss staining (red). Expression of LacZ by ato-Gal4 (green) and expression of GFP from the fussMi13731/+ reporter line (red). Labelling of Acj6 with anti-Acj6 antibody (green) and expression of CD8-GFP with fussBD-Gal4 (red). Scale bars indicate 10 μm. (C) log2(Dam-PolII/Dam) data from controls compared with log2(Dam-PolII/Dam) data from Fuss mutant neurons show only small deviations from each other. Coefficient of determination R2 = 0.889. (D) TaDa reveals sensory neuron marker expression of EAAT2, Ir76a, and Gr66a in both datasets (upper lane: control; lower lane: mutant) with a clear reduction in PolII coverage of Gr66a in the mutant dataset in contrast to the control dataset. PolII coverage is depicted under PolII binding pattern. Regions bound stronger by Dam-PolII than by Dam are depicted in green. Regions bound stronger by Dam than by Dam-PolII are depicted in red.