CD8+ T cells that survive depleting mAb treatment acquire distinct differentiation phenotypes.

(A) Flow cytometry gating strategy: after gating on singlet and live lymphocytes, B cells were gated away and the CD45.1+ adoptively transferred OT1 T cells were gated on. Subsequently OT1 T cells were assessed for IL-7Rα and KLRG1 expression. (B) Percent of OT1 T cells that are of MPEC phenotype (IL-7Rα+ KLRG1-) and gMFI of IL-7Rα expression of the MPEC cell only. (C) % of OT1 T cells that are SLEC phenotype (IL-7Rα- KLRG1+) and gMFI of KLRG1 of the SLEC cells only. Representative data shown from 3 independent experiments for immunization and 2 experiments for infection. Each point represents the average value of a mouse with 3–4 mice per group.