CD8+ T cells that survive depletion participate in immune responses.

(A-B) 106 CD45.1 OT1 T cells were transferred i.v. into CD45.2+ mice, treated i.p. with 500μg of anti-CD8α or –β the next day, and immunized or infected. Spleens and lymph nodes were collected on day 5 post-infection or on day 7 post-immunization. CD8+ T cells were considered as CD45.1+ B220-. (A) Surface bound depleting mAb on OT1s was determined by staining with Goat anti-Rat IgG. (B) Remaining OT1 T cells as a percentage of lymphocytes and total numbers were calculated per spleen or pooled lymph nodes. Data representative of at least 3 independent experiments. Each point represents the average value of a mouse with 3–4 mice per group.