Confocal depth image series of <i>in planta</i> cellular phenotype of <i>E</i>. <i>festucae</i> WT and <i>gpiB</i>, <i>sspM</i>, <i>sspN and sspO</i> OE strains.

Infected L. perenne pseudostem samples were stained with aniline blue/WGA-AF488. Hyphae show aniline blue fluorescence of β-glucans (red pseudocolor) and chitin staining of septa with WGA-AF488 (blue pseudocolor). (A) Representative growth of WT and OE strains in planta (z = 15 μm); Bar = 20 μm. (B) Magnification of the area boxed with white dashed lines in A, showing representative growth of the strains in more detail (z = 15 nm); Bar = 20 μm. (C) Hyphal branching and fusion of WT and OE strains in planta (z = 5 nm); Bar = 20 μm.