Comparative infection analyses of <i>C. lindemuthianum</i> and <i>Glomerella</i> strain on tissues of the Pérola susceptible cultivar.

<p>A) <i>C. lindemuthianum</i> tQB02 transformant strain on a hypocotyl at 72 hpi. White arrows indicate appressoria, and the black arrow indicates the location of primary hyphae. B) Same view as in panel A, imaged with fluorescence microscopy showing primary hyphae expressing GFP (white arrows). Bar: 20 µm. C) <i>Glomerella</i> tQB01 transformant strain inoculated on a hypocotyl at a site where cell death had been induced. Thick hyphae were observed inside the dead cells by 48 hpi (white arrows). D) Same view as in panel C, imaged with fluorescence microscopy, showing thick hyphae expressing GFP (white arrows). Bar: 20 µm. E) <i>Glomerella</i> tQB01 transformant strain forming acervuli and spores (white arrows) at 72 hpi on a leaf that had tissue been killed by treatment with dry ice. F) Same view, imaged with fluorescence microscopy showing spores (white arrows). Bar: 20 µm. G) Merged image taken with light and fluorescence microscopy showing co inoculation on hypocotyl using transformant teleomorphic strain (tQB01) and wild type anamorphic strain (LV115). tQB01 expressing GFP on hyphae (white arrow) growing on anthracnose lesion at 120 hpi. Bar: 20 µm. H) Co inoculation on hypocotyls at 120 hpi using the transformant <i>C. lindemuthianum</i> strain (tQB02) and the perithecial wild type <i>Glomerella</i> strain (UFLAG06). Appressoria of the UFLAG06 strain have formed around the lesion (white arrows). I) Same image taken by fluorescence microscopy showing hyphae of the tQB02 strain expressing GFP in the lesion (white arrows). Bar: 20 µm.