GTSF-1 is required for sRNA biogenesis and target silencing in adult males.

(A-E) Normalized levels of sRNAs in RppH treated libraries, in RPM. Three biological replicates are shown. WT, wild-type. (A) Total levels of 26G-RNAs. (B) 26G-RNAs mapping to ERGO-1 targets. (C) 26G-RNAs mapping to ALG-3/4 targets. (D) total levels of 21U-RNAs. (E) total levels of 22G-RNAs. (F) RPM Levels of sRNAs mapping, per gene, to known targets of ALG-3/4 and ERGO-1. (G) Normalized mRNA expression of ALG-3/4 and ERGO-1 targets, in RPKM. Violin plots in (F-G) show the distribution density of the underlying data. The top and bottom of the embedded box represent the 75th and the 25th percentile of the distribution, respectively. The line in the box represents the median. P-values were calculated either with a two-sided unpaired t-test (A-E), or with a two-sided Mann-Whitney/Wilcoxon rank-sum test (F-G).