Small RNA formation from control and siRNA sensor transgenes.

<p>(A) Size and 5′ nt distributions of GFP-derived small RNAs deep sequenced from <i>C. elegans</i> containing control or siRNA sensor transgenes. (B) Normalized reads (reads per million total reads) mapping to GFP mRNA from control- and siRNA sensor-transgenic <i>C. elegans</i> deep sequencing libraries. (C) Small RNA distribution across the control GFP transgene. (D) Small RNA distribution across the siRNA sensor transgene. Inset, RNA blot assay for 22G siR-1 from control- and siRNA sensor-transgenic <i>C. elegans</i>. EtBr stained tRNAs are shown as a loading control.