Quantitation of apoptosis and necrosis by flow cytometry in CsA-treated renal tubular cells: The effect of the restoration of GADD45γ expression in GADD45γ knockdown cells.
- Gyu-Tae Shin (5819249)
- Hwa Joung Lee (6391901)
- Ji Eun Park (455778)
Publication date
February 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
shRNA-GADD45γ cells were infected with 50 MOI of Ad-LacZ or Ad-GADD45γ for 48 hours and then treated with 25 ug/ml CsA for 72 h. (A) Representative scatter plots of flow cytometry. Quantitation of (B) apoptotic cells (PI negative and Annexin V positive, Q4), (C) necrotic cells (PI positive and Annexin V positive, Q2), (D) viable cells (PI negative and Annexin V negative, Q3). Each bar represents mean ± SD of 3 experiments. *p < 0.05 vs Ad-LacZ transduced cells (Student's t-test). Ad-GADD45γ(G) represents adenoviral vectors expressing GADD45γ; Ad-LacZ, LacZ adenoviral vectors.</p
Add to ORKG