Additional file 4: of Paeniclostridium sordellii and Clostridioides difficile encode similar and clinically relevant tetracycline resistance loci in diverse genomic locations

Figure S2. Nucleotide alignment of the upstream regulatory regions of Tet P in P. sordellii and C. difficile isolates compared to that of C. perfringens pCW3 from strain CW92. The sequence from P. sordellii isolates that encode Tet P on small plasmids is represented by R28058 (but includes R15892, SSCC37615, R32977 and SSCC19939, Table S1). The sequence from P. sordellii isolates that appear to encode Tet P on the chromosome, with the exception of W2945, is represented by SSCC18392 (but includes SSCC32135, R32462, JGS6961, W3026, AM370 and W2922, Table S1). The beginning of the tetA(P) gene is colored red (red arrow) with the start codon in bold. The predicted ribosome binding sites (RBS) for these sequences are in black bold font. The region corresponding to the C. perfringens pCW3 Tet P promoter, P3, is highlighted in green with the predicted − 10 and − 35 boxes shown in bold. The inverted repeats that correspond to the T1 transcriptional terminator are highlighted in blue and indicated with inverted black arrows. (PDF 274 kb