Identification of cDC subsets that take up OVA and migrate to draining tLNs.

The experimental design corresponds to that of Figs 9 and 10, using fluorescence-labeled OVA to restrict the analysis to cells that have captured OVA in airway mucosa and migrated to draining tLNs. (A) Gating strategy to distinguish between Ly6clow cDCs and Ly6chigh MoDCs, as well as between cDC subsets based on mutually exclusive expression of CD11b and CD103, defining CD11b+ cDCs and CD103+ cDCs, respectively. (B) Relative and absolute quantitations of OVA-laden Ly6clow cDCs and Ly6chigh MoDCs present in tLNs after airway sensitization to OVA alone (OVA) or combined with mCMV (mCMV + OVA). (C) Relative and absolute quantitations of OVA-laden CD11b+ cDCs and CD103+ cDCs present in tLNs after airway sensitization to OVA alone (OVA) or combined with mCMV (mCMV + OVA). Symbols in (B) and (C) represent data from individual mice with mean values marked. Data were compiled from 2 independent experiments with altogether 6–7 mice per experimental group. Asterisk-coded statistical significances: *P≤0.05; **P≤0.01; ***P≤0.001; n.s.: not significant.