OVA-specific Ab responses in systemic and mucosa-associated lymphoid tissues of chimera mice which lack CCR5- or CCR6-expressing DCs (Figure S1).

<p>(A and C) CD11c-DTR, CD11c-DTR/C57BL/6, CD11c-DTR/CCR5^−/− and CD11c-DTR/CCR6^−/− mice were injected with DT via the intraperitoneal route 6 h before each nasal immunization (three times at weekly intervals with OVA plus Ad-FL). CD11c-DTR/C57BL/6 chimera mice given the DT injection served as positive controls. (B) CD11c-DTR/C57BL/6, CD11c-DTR/CCR5^−/−, CD11c-DTR/CCR6^−/− and normal C57BL/6 were nasally immunized three times at weekly intervals with OVA plus Ad-FL without DT injection. (A and B) Seven days after the last immunization, levels of SIgA anti-OVA Abs in saliva, SIgA and IgG Abs in NWs, and IgA, IgG and IgM Abs in plasma were determined by OVA-specific ELISA. (C) MNCs from NPs, SMGs and spleens were isolated 7 days after the last immunization and subjected to OVA-specific ELISPOT assay to determine the numbers of IgA, IgG and IgM AFCs. The values shown are the mean ± SEM taken from five separate experiments with a total of 25 mice in each experimental group. The data for controls were obtained from two separate experiments which consisted of 6 mice for each experiment. The ELISA and ELISPOT data for spleen represented the Ab responses from 12 individual mice for controls and 25 individual mice for experimental groups. MNCs from SMGs and NPs were pooled from 2 or 3 mice and subjected to OVA-specific ELISPOT assays. N.D. means that O.D. values were not detected. (A and C) *<i>p</i><0.05 when compared with DT treated, CD11c-DTR/C57BL/6 chimera mice nasally immunized with OVA plus Ad-FL. (B) No significant differences were detected when compared with normal C57BL/6 mice.