Additional file 4: of Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome

Figure S1. Vectorette PCR amplicons quality control. a. Electrophoresis of vectorette PCR amplicons. The gel image shows five lanes: [L] 2-log ladder (NEB) [1], 2 μg purified PCR amplicons [2], 2 μg purified PCR amplicons digested with BstYI [3], 2 μg purified PCR amplicons ‘contaminated’ with concatemers [4], 2 μg purified PCR amplicons digested with BstYI showing concatemer band at ~ 50 bp. A good vectorette PCR will present as a smear of amplicons averaging 1-3 kb (lane 1). A very high molecular weight smear could indicate contamination (lane 3). b. Schematic of possible vectorette-primer concatemer. Digestion of the amplicons with BstYI, which cuts inside the vectorette primer sequence, will produce a visible band at ~ 50 bp if concatemers are in the PCR product. (PDF 2479 kb