Role of Hsf1 in the induction of mating projections in <i>C</i>. <i>albicans</i>.

(A) Morphologies of the control and tetON-HSF1/hsf1 mutant. 1 × 105 cells were spotted on YPD-K and YP-K media without or with 40 μg/mL Dox and cultured at 25°C for three or five days. Percentages of projected cells are indicated in the corresponding images. Scale bar for colonies, 2 mm (inset); scale bar for cells, 10 μm. Control, GH1013cartTA. (B) Relative expression levels of mating-related genes. 1 × 105 cells of each strain were cultured on YPD-K medium (for six days) or on YP-K medium (for three days) without or with 40 μg/mL Dox at 25°C. Error bars represent standard errors of technical duplicates. *p t test. Experiment was repeated in a biological replicate, and a representative image is shown. The numerical data are presented in S3 Data. Dox, doxycycline; FIG1, Factor-Induced Gene 1; FUS1, cell FUSion 1; Hsf1, Heat Shock transcription Factor 1; MFA1, Mating type A1; MFα, Mating α factor precursor; p, mating projection; tetON, tetracycline-induced; tetON-HSF1/hsf1, tetON-promoter–controlled conditional expression strain of HSF1; WT, wild type; YPD-K, yeast extract-peptone-glucose-K2HPO4; YP-K, yeast extract-peptone-K2HPO4.