Global gene-expression–profile analysis of <i>C</i>. <i>albicans</i> in the presence and absence of glucose.

C. albicans cells were spotted and grown on YPD-K or YP-K media at 25°C for 60 hours. Total RNA was extracted and used for RNA-Seq assays. To be considered differentially expressed, a gene must satisfy three criteria: (1) an FPKM value higher than or equal to 20 at least in one sample, (2) a fold change value higher than or equal to 1.5, and (3) an adjusted p-value (FDR) lower than 0.05. (A) Venn diagram depicting relationships between differentially expressed genes on YPD-K (412) and YP-K (408) media and HSP90 genetic interactors (indicated in the ellipse). (B) Selected heat-shock-protein–encoding, oxidative-stress–induced, and mating-related genes up-regulated in YP-K medium. AIF1, Apoptosis-Inducing Factor 1; BAR1, BARrier 1; CEK1, Candida ERK-family protein kinase; ERK, ERK-family protein kinase; FDR, false discovery rate; FPKM, fragments per kb per million reads; GPI, glycosylphosphatidylinisotol; GST3, Glutathione S-transferase; HMX1, HeMe oXygenase; Hsp90, Heat shock protein 90; KAR2, KARyogamy; MAP, mitogen-activated protein; MFA1, Mating type A1; NADH, Nicotinamide adenine dinucleotide; orf19.3475, Candida gene orf19.3475; PST2, Protoplasts-SecreTed 1; RNA-Seq, RNA sequencing; SIS1, Slt4 Suppressor; SOD5, SuperOxide Dismutase 5; SSA2, Stress-Seventy subfamily A; STE4, STErile 4; YPD-K, yeast extract-peptone-glucose-K2HPO4; YP-K, yeast extract-peptone-K2HPO4.