Additional file 6: of Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

Figure S4. FACS gating strategy used to collect EGFP positive cells from a clutch of Tg(fli1a:EGFP) zebrafish. Representative samples of a negative control sample (left) and a Tg(fli1a:EGFP) sample (right). This gating strategy was used to first select live cells based on forward and side scatter, with subsequent selection of single cells. Last, GFP positive cells were selected and sorted. No GFP+ cells are seen in control fish (left) while 5–15% of the cell population was found to be GFP positive cells in Tg(fli1a:EGFP) samples (right) (PDF 1027 kb