Effects of acetaldehyde (AcH) and phosphatidylethanol (PEth) on phenylephrine-induced constriction of mesenteric resistance arteries.

Pressure myography experiments with arteries pressurized to 80 mmHg. Data are mean ± SEM. A. AcH concentrations (μM) in the vessel bath during the time frame of the experimental procedure (30 min pretreatment followed by stimulation with increasing doses of PE), n = 6 for each concentration. B. PE-induced constriction in % of the o.d. in arteries pretreated with AcH for 30 min. Vehicle—n = 4; 0.1 μM AcH–n = 3; 1 μM AcH–n = 4; 5 μM AcH–n = 4, 10 μM AcH–n = 3. C. EC50 concentrations for the vasoconstrictor effects of PE (nM) from dose-response curves in B. *: pD. PEth concentrations (μM) in the vessel bath during the time frame of the experimental procedure (30 min pretreatment followed by stimulation with increasing doses of PE), n = 6–9. E. PE-induced constriction in % of the o.d. in arteries pretreated with PEth for 30 min. Vehicle—n = 4; 0.25 μM PEth–n = 4; 1 μM PEth–n = 4; 2.1 μM PEth–n = 5. F. EC50 concentrations for the vasoconstrictor effects of PE (nM) from dose-response curves in D.