Pre-autophagosomal membranes fail to coalesce in <i>TMEM41B</i>^<i>KO</i> cells.

CLEM of HEK293T TMEM41BKO (A and B) and wild-type HEK293T cells (C) expressing tfLC3 that were grown under basal conditions (no drug treatment). Analysis workflow is indicated by green arrows. White arrowheads indicate a representative structure of interest. White boxes demarcate zoomed areas in subsequent images. Blue indicates Hoechst 33342. Panel A includes a sample tomogram used to make the three-dimensional reconstruction (in B) of the membranous structures found in the GFP-positive region. Green indicates membrane sheets and tubes; cyan indicates membrane vesicles. See Materials and methods for further CLEM analysis details. Related to S6 Fig. CLEM, correlative light and electron microscopy; GFP, green fluorescent protein; HEK, human embryonic kidney; LC3, microtubule-associated protein 1 light chain 3B; TEM, transmission electron microscopy; tf, tandem-fluorescent.