(A and F) Recombinant protein production of FLAG-tagged versions of Ol-bsf and Ol-cug-bp1 and western blotting detection with an anti-FLAG antibody. (B–E and G–I) EMSA using the recombinant medaka Ol-bsf and Ol-cug-bp1 proteins (in vitro translated) and either the medaka D3U-box sequence (B, C, E, G, H, I), or the Drosophila D3U-box sequence (D) as radioactively labelled RNA probes. Increasing amounts of unlabelled probes (scrambled [B, I], medaka [C, G, K], Drosophila [D, E], minus CUG [H, L] target sequences) were used as competitors. (B–E and G–I) Apparent shifts are observed for the labelled RNA target probes with the Ol-bsf and Ol-cug-bp1 RNA-binding proteins, likely indicating direct interactions. Ratios of RNA probe to RNA competitor...
<p>(a) Lane 1, <sup>32</sup>P-labeled MSgt-FSgt probe only; lane 2–3, <sup>32</sup>P-labeled MSgt-FS...
<p>(A) Sequences of DNA probes used in EMSA. DR probe contains four copies of 15-bp repeats from nt ...
<p>(A) & (B) The targeted regions on the RNAs for each of the snoMEN vectors used in this study are ...
<p>(<b>A</b>) Overview of oligonucleotides used for EMSA. LKB1 DSE: downstream element, position +35...
A. Direct EMSA with the following probes: tamA, pckA, bvrR, omp25, virB1, and negative controls rplL...
<p><b>Panel (A)</b>: Shows the EMSA experiments to evaluate the specificity of the Oligo – Nuclear p...
<p>Interactions were examined by electrophoresis mobility shift assay (EMSA). (A) Nucleotide sequenc...
<p>(A) A 96 bp fragment from the ALF85+ promoter was labeled and used as the probe in EMSA assays wi...
<p>(A) Lanes 1 and 2 indicating the mobilities of labeled, biotinylated oligonucleotides (A33 and G3...
<p>A. EMSA experiments with protein extracts from S2 cells and adult <i>D</i>. <i>melanogaster</i> f...
<p><b>Panel (A)</b>: Shows the EMSA experiments carried out to assess the displacement of the probe ...
<p>S2 cells were transfected to co-express sense and antisense transcripts of LacZ to silence LacZ e...
<p><b>transposase to its ITR sequences</b>. (<b>A</b>) EMSA assay. Detection of the binding of Hsmar...
<p>(A) Binding of GFP-L22 to stem-loop III of EBER-1 (SL3) was tested in RNA EMSA experiments using ...
<p>(<b>A</b>) Flag (dark green) bound to a neutravidin (yellow) with Cy3B-conjugated (red) oligonucl...
<p>(a) Lane 1, <sup>32</sup>P-labeled MSgt-FSgt probe only; lane 2–3, <sup>32</sup>P-labeled MSgt-FS...
<p>(A) Sequences of DNA probes used in EMSA. DR probe contains four copies of 15-bp repeats from nt ...
<p>(A) & (B) The targeted regions on the RNAs for each of the snoMEN vectors used in this study are ...
<p>(<b>A</b>) Overview of oligonucleotides used for EMSA. LKB1 DSE: downstream element, position +35...
A. Direct EMSA with the following probes: tamA, pckA, bvrR, omp25, virB1, and negative controls rplL...
<p><b>Panel (A)</b>: Shows the EMSA experiments to evaluate the specificity of the Oligo – Nuclear p...
<p>Interactions were examined by electrophoresis mobility shift assay (EMSA). (A) Nucleotide sequenc...
<p>(A) A 96 bp fragment from the ALF85+ promoter was labeled and used as the probe in EMSA assays wi...
<p>(A) Lanes 1 and 2 indicating the mobilities of labeled, biotinylated oligonucleotides (A33 and G3...
<p>A. EMSA experiments with protein extracts from S2 cells and adult <i>D</i>. <i>melanogaster</i> f...
<p><b>Panel (A)</b>: Shows the EMSA experiments carried out to assess the displacement of the probe ...
<p>S2 cells were transfected to co-express sense and antisense transcripts of LacZ to silence LacZ e...
<p><b>transposase to its ITR sequences</b>. (<b>A</b>) EMSA assay. Detection of the binding of Hsmar...
<p>(A) Binding of GFP-L22 to stem-loop III of EBER-1 (SL3) was tested in RNA EMSA experiments using ...
<p>(<b>A</b>) Flag (dark green) bound to a neutravidin (yellow) with Cy3B-conjugated (red) oligonucl...
<p>(a) Lane 1, <sup>32</sup>P-labeled MSgt-FSgt probe only; lane 2–3, <sup>32</sup>P-labeled MSgt-FS...
<p>(A) Sequences of DNA probes used in EMSA. DR probe contains four copies of 15-bp repeats from nt ...
<p>(A) & (B) The targeted regions on the RNAs for each of the snoMEN vectors used in this study are ...