Detection of interactions between FMTRIP-hybridized mRNA and cytoskeletal elements using proximity ligation assay (PLA).

<p>(<b>A</b>) Flag (dark green) bound to a neutravidin (yellow) with Cy3B-conjugated (red) oligonucleotides (red dash) formed an FMTRIP. (<b>B</b>) Streptolysin O created entrance for FMTRIP in the cell membrane, allowing access to the mRNA (gray) bound to the β-tubulin (blue), vimentin (magenta), and F-actin (green), via RNA-binding proteins (RBP, brown). (<b>C</b>) Antibodies against the flag (light blue) and the cytoskeletal element (light magenta) in addition to the proximity probes against the antibodies (dark blue and magenta) attached to the FMTRIP-bound mRNA (gray) and the cytoskeleton (green); the oligonucleotides (black dash) on the proximity probes join to synthesize a Cy5-equivalent hybridized DNA product (light green and black dash) via rolling circle amplification.