ISG expression is generally increased in IFITKO mice at 48 hours after CVB3 infection.

B6 and IFITKO mice (n = 3 per group) were infected with 104 pfu of CVB3, and were sacrificed 48 hours later. Pancreata, livers and hearts were harvested, RNA was extracted and the expression of 84 ISGs was analyzed using a mouse T1IFN response profiler PCR array. RNAs from organs of uninfected mice were used as controls. (A) Heat maps showing the fold gene induction (compared to uninfected tissues) in the three organs from infected B6 and IFITKO mice. The IFIT genes (negative in the heat maps for the IFITKO mice) are enclosed in a dashed gold box. (B) To more easily visualize the overall expression patterns in the B6 and IFITKO organs, the same data are graphed using a violin plot. Blue line = median; red lines = 25th and 75th quartiles. Because they are, by definition, absent from IFITKO mice, the IFIT genes were excluded from these violin plots; thus, these plots compare the expression of 81 ISGs in B6 and IFITKO mice.