SPP and SKI-1 inhibitors block entry driven by diverse filovirus glycoproteins in a concentration and time-dependent manner.

(A) 293T cells were incubated with the indicated inhibitors or DMSO as control and transduced with rhabdoviral particles bearing the indicated viral glycoproteins. Transduction efficiency was analyzed by determining luciferase activities in cell lysates at 24 h post transduction. The results of a representative experiment performed with triplicate samples are shown. Error bars indicate standard deviation. Similar results were obtained in a separate experiment (SD). C.p.s., counts per second. (B) Rhabdoviral vectors pseudotyped with the indicated filovirus glycoproteins were used for transduction of target 293T cells pretreated with the indicated inhibitors or DMSO as a control. Transduction efficiency was analyzed by determining luciferase activities in cell lysates at 24 h post transduction. The average of three independent experiments performed with triplicate samples is shown. Transduction mediated by particles bearing EBOV-GP (GP from Mayinga variant, EBOV’76) was set as 100%. Error bars indicate standard error of the mean (SEM). (C) 293T cells were treated with the indicated inhibitors at the indicated time points and transduced with rhabdoviral vectors pseudotyped with the indicated viral glycoproteins. Transduction efficiency was analyzed by determining luciferase activities in cell lysates at 24 h post transduction. The average of three independent experiments performed with triplicate samples is shown. Transduction measured in the presence of DMSO was set as 100%. Error bars indicate standard error of mean (SEM). Asterisks indicate a statistical significant decrease (* = p<0.05; ** = p<0.01; *** = p<0.001) of transduction in the presence of inhibitor as compared to DMSO.