Impact of media condition on identification of consensus essential genes.

(A and C). Venn diagrams of candidate essential gene lists for transposon mutagenesis screens performed on LB for PAO1 and PA14, respectively. (B and D). Overlap analysis of candidate essential gene lists for transposon mutagenesis screens performed on LB for PAO1 and PA14, respectively. Blue bars indicate the total number of candidate essential genes identified in each screen. Black bars indicate the number of candidate essential genes unique to the intersection given by the filled-in dots. The orange bar indicates the overlap for all screens for either PAO1 (Panel B) or PA14 (Panel D). The black and orange bars correspond to the intersections identified in the venn diagrams in panels A and B.