Stringent cross-species comparison of gene expression changes in B-cell lymphoma discovers 60 concordantly deregulated genes, designated DMB (DLBCL/iMycBCL).

<p>(A) The Venn diagram on the left shows the degree of human-in-mouse overlap of gene probesets found to be significantly variable in both species. Results at FDR threshold values of 5% and 1% are indicated in blue and red, respectively. A heat map of unsupervised cluster analysis of matching human-mouse gene sets at FDR 0.05 is depicted on the right. (B) Degree of mouse-in-human overlap of significantly variable gene probesets in both species, using the same approach as in panel A. Panels A and B depict reciprocal results. (C) Venn diagram indicating that overlapping gene sets from panels A and B (FDR 0.01 in both cases) represent 130 concordantly deregulated genes in human DLBCL and mouse iMycBCL. (D) Diagrammatic representation of two filtering steps that narrowed the gene list from 130 genes to 60 genes. The genes eliminated in this process are indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076889#pone.0076889.s004" target="_blank">Table S1</a>. (E) Column diagram indicating the top five gene ontology (GO) categories for the 130 concordantly deregulated genes from panels C and D left. GO categories were determined using DAVID. Pathway names and numbers are shown to the right.