Measurement of ER stress/UPR levels in mutant sciatic nerves at P28.

(A-C) Western blot analysis for the ER stress/UPR markers BiP, GRP94 and P-eIF2α on P28 sciatic nerve lysates; β-Tubulin was used as loading control. One representative blot of four is shown. (D-F) Protein levels of BiP, GRP94 and P-eIF2α as measured by densitometric analysis. (G-I) qRT-PCR analysis on P28 sciatic nerve extracts for BiP, CHOP and spliced Xbp1; WT animals were used as the reference group. n = 4–5 RT from independent pools of three nerves per genotype. Error bars, SEM; *P t test.