(A and B) Western blot analysis of Ski (A) and Rb and pRb (pRb; B) expression in developing rat sciatic nerves, and graphs representing the densitometry of the bands normalized to the loading control β-actin or GAPDH

Statistical analyses were performed using two-tailed tests on three groups of animals for each age. (C and D) Images of single confocal sections of Ski (red; C) or pRb (pRb; green; D) and DAPI (blue) immunostainings on longitudinal sections of P0 and adult rat sciatic nerves. Arrows point out Schwann cell nuclei. (E) Images of single confocal sections of Ski (red) and pRb (pRb; green) coimmunostainings and DAPI (blue) labeling on teased nerve fibers of P0 and adult rat sciatic nerves. In C–E, Ski appears pink, and pRb appears turquoise when nuclear. In E, the overlay of Ski and pRb appears yellow. Arrows indicate examples of the colocalization of Ski and pRb. Insets are magnifications of the regions outlined by boxes. (F) Immunoprecipitation of Ski and Western blot of pRb (pRb) or Ski in the cytoplasmic (C) and nuclear (N) fractions of P7 and adult rat sciatic nerve lysates. Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.<p><b>Copyright information:</b></p><p>Taken from "Expression and localization of Ski determine cell type–specific TGFβ signaling effects on the cell cycle"</p><p></p><p>The Journal of Cell Biology 2008;182(3):519-530.</p><p>Published online 11 Aug 2008</p><p>PMCID:PMC2500137.</p><p>