(A–C) Images of single confocal sections of the coimmunostaining of Ski (red) and Rb (green; A) or Ski (red) and pRb (pRb; green; B) in rat Schwann cells or in WB-F344 cells (C) cultured in DM or treated with TGFβ (the overlay appears yellow)

Arrows indicate examples of the colocalization of Ski and Rb (A) and Ski and pRb (B). Insets are magnifications of the regions outlined by boxes. (D) Immunoprecipitation of Ski and Western blotting of Rb or Ski in the cytoplasmic (C) and nuclear (N) fractions of rat Schwann cells treated with TGFβ for 36 h, and Western blot of lamin (nuclear marker) and GAPDH (cytoplasmic marker) performed on lysates used for immunoprecipitation (input). The arrow indicates a nonspecific band or a degradation product.<p><b>Copyright information:</b></p><p>Taken from "Expression and localization of Ski determine cell type–specific TGFβ signaling effects on the cell cycle"</p><p></p><p>The Journal of Cell Biology 2008;182(3):519-530.</p><p>Published online 11 Aug 2008</p><p>PMCID:PMC2500137.</p><p>