The direction of conditioning along the frequency axis affected learning, but not generalization.

(A-C) Comparison between mice trained with different direction of conditioning (conditioned tone was higher or lower than safe) along the frequency axis. Baseline (black) and average responses to the safe (blue) and conditioned (pink/red) tones during the last 50 conditioned visits in the conditioning phase. All mice in a plot were trained with the same pair of stimuli, (A) 7000 Hz and 14000 Hz tone, (B) 5885 Hz and 11770 Hz tone, and (C) 7000 Hz and 11770 Hz tone. (D) Mean d’ during the last 50 conditioned visits in the conditioning phase. Stars: p = 0.004 2-sample t-test (E-G) Fitted psychometric curves and thresholds (vertical line) for mice described in (A-C), respectively. Black dots indicate outliers in the fitting (value above or below 1.5 times the standard deviation).