Results in the two-tone discrimination task of eyeblink conditioning and the rotor rod test in DCN-lesioned and sham-operated mice (Exp. 2b).

<p>(A) HCS+ (10 kHz) preceded and co-terminated with the US, and the LCS− (1 kHz) was presented without the US. (B) (C) Results of CR% in HCS+ and LCS− trials are shown for sham-operated (B) and DCN-lesioned mice (C). In sessions 1 and 2, basal eyeblink responses were recorded without tone CS and electric shock US (Hab. means the habituation sessions.). All mice received two-tone discrimination task for 10 days (sessions 3–12). CR% was calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059880#pone-0059880-g004" target="_blank">Fig. 4</a>. (D) (E) Normalized EMG amplitude profiles measured in HCS+ and LCS− trials of session 12 in sham-operated (D) and DCN-lesioned mice (E). Arrowheads indicate the onset of the CS. EMG amplitudes of valid trials were normalized in HCS+ and LCS− trials. (F) (G) Histograms of normalized EMG amplitudes in CR trials binned into 50 ms in the session 12. Normalized EMG amplitudes in CR trials were averaged in HSC+ and LSC− trials in sham-operated mice (F) and DCN lesioned mice (G). (H) Averaged fall latencies in four trials are shown for the daily sessions. *indicates <i>P</i><0.05 (HCS+ vs. LCS−); **indicates <i>P</i><0.01 (sham-operated vs. DCN-lesioned).