Serum growth characteristics of candidate fitness factor mutants.

Growth of wild-type P. mirabilis and mutants over the course of 24 hours during static incubation at 37°C with 5% CO2 in 50% naive mouse serum for genes that are (A) predicted to have a defect in serum (asnA, cvpA, hflK) or (B) candidate infection specific fitness factors (arnA, btuB, cutC, gltB, speA, tatC). Cultures were sampled at 0, 1, 3, 6, and 24 hours for enumeration of CFUs on LB agar. Error bars represent the mean ± standard deviation from three biological replicates.